Environmental Surface Sampling Protocol

Step-by-Step Guide for Accurate Sample Collection

Follow these steps to ensure reliable test results using aseptic techniques, gloves, and 3M sterile swabs.

1. Prepare for Sampling

Wear clean gloves and gather sterile 3M swabs and the Chain of Custody (COC) form.
✅ Identify the food contact surface or environmental location to be swabbed.

2. Open the Swab Properly

Tear open the sealed packaging carefully.
✅ Hold the handle—🚫 do not touch the sponge or the area below the finger stop.

3. Swabbing Process

🔹 Horizontal Stroke: Swab the surface once horizontally with firm, even pressure.
🔹 Vertical Stroke: Rotate the swab and swab once vertically on the same area.

4. Secure the Sample

Return the swab to its sterile bag without touching unintended surfaces.
✅ Bend the handle from outside the bag to detach it—🚫 do not reach inside the bag.
✅ Seal the sample bag by folding and securing the wire tabs.

5. Complete the COC Form

Record:

  • Location, date, and time of sample collection.
  • Area description (e.g., equipment name, zone).
  • Signature in the “Relinquished By” section.

6. Transport the Sample

Place the sealed sample bag in a cooler with ice packs.
✅ Include a paper copy of the COC form in the shipment box.
✅ Ship immediately to maintain sample integrity.

📞 Need assistance? Contact QuanTEM Food Safety Laboratories.